2BIO035 Bioprocess Technology
- Course code2BIO035
- Number of credits5
- Teaching semester2024 Spring
- Language of instruction and examinationEnglish
- CampusHamar
- Required prerequisite knowledge
None
Content
- production of biologicals in the bioprocess/ biopharmaceutical industry
- fermentation technology
- microbial and mammalian cell cultures
- genetic engineering; cloning methods, vector systems, recombinant protein production
- protein isolation and separation methods; centrifugation, solubility, chromatography and membrane separations
- process economy considerations
- structured experimental approuch by Design of Experiments
Learning outcome
Upon successful completion of the course, the student will have achieved the following learning outcomes:
The student
- has thorough knowledge of the underlying principles of main bioprocess unit operations like fermentation, downstream processing
- has thorough knowledge about genetic engineering for recombinant protein expression and production from various cell systems
- has advanced knowledge about factorial experimental set up (Design of Experiments)
The student
- understands how lab scale methods transform into large scale, and how main unit operations in downstream processing (centrifugation, filtration, and chromatography) function
- has advanced understanding of design of expression vector systems for protein production
- can perform experimental procedures for recovery and purification of a protein and evaluate the outcome
The student
- understands how and why bioprocessing is employed to increase value of the feed stream
- has deeper knowledge of the main workflow and logic behind bioprocessing both in lab and industrial scale, and able to identify unit operations
- can analyze how genetic engineering is used to harness cell systems for protein production
Organization and Methods of Instruction
- lectures ca. 20 hours
- lab exercise - three days
- in-class group activities
Requirements necessary to take the exam
- 100% attendance at the preparatory lab lecture
- 100% participation at laboratory exercise
- one written individual laboratory report from lab must be passed
| Form of assessment | Grading scale | Grouping | Duration of assessment | Support materials | Proportion | Comments |
|---|---|---|---|---|---|---|
Written examination with supervision | ECTS - A-F | Individual | 4 Hour(s) |
| 100 % |
Reading list
No reading list available for this course