BIOING1202 Molecular biology and genetics

    • Studiepoeng
      10
    • Undervisningssemestre
      2023 Høst
    • Undervisningsspråk
      Norsk/Engelsk
    • Undervisningssted
      Hamar
    • Forkunnskapskrav

      Studenten må normalt ha fullført minst 45 studiepoeng i løpet av det første studieåret i bachelor bioingeniørfag

Emnets innhold

Course content

  • structure and function of RNA and DNA (both chromosomal and extrachromosomal), nucleoid, chromatin, chromosomes (organellar, and nuclear - both interphase, mitotic and meiotic) and extrachromosomal DNA 
  • chromosomal and extrachromosomal DNA replication, mutation, repair and recombination (site-specific, homologous, meiotic and somatic)
  • prokaryotic and eukaryotic transcription and regulation thereof, including RNA processing in eukaryotes
  • regulation of translation in prokaryotes and eukaryotes
  • regulation of replication, transcription and translation of viruses that utilise prokaryotic or eukaryotic hosts
  • genetic mutation, polymorphism, variation and inheritance, pedigree analysis, human Mendelian genetics, chromosomal aberrations and deviant states of ploidy
  • recombinant DNA technology (including cloning), genetic engineering, genetically modified organisms, DNA sequencing, polymerase chain reaction (PCR, also quantitative PCR, QPCR), gene editing and gene therapy
  • genome organisation, genomics, functional genomics, proteomics, transcriptomics
  • epigenetics -- histone modification, DNA methylation, X-inactivation, DNA imprinting; prions and Creutzfeldt-Jakob disease
  • regulations for work with biological material and GMO
  • laboratory exercises, including nucleic acid extraction, quantitation, restriction, PCR-amplification (end point, semiquantitative and quantitative), dissociation, hybridisation sequencing and cloning; report writing with an emphasis on the analysis and dissemination of results and conclusions from analyses

Læringsutbytte

Upon passing this course, the graduate will have achieved the following learning outcomes:

Kunnskap

The student

  • has knowledge of the content, meaning and expression of genetic information from DNA to protein
  • has knowledge of the regulatory mechanisms controlling DNA replication, transcription and translation in humans and organisms (including viruses) relevant for human health
  • has knowledge about all forms of mutation of nucleic acids, how they arise, their effects on gene expression and how they are repaired
  • has knowledge of the principles of genetic inheritance, pedigree analysis and how mutant alleles are inherited and how they can affect phenotypes including causing disease
  • has knowledge about different forms of polymorphisms in DNA and how they can be developed and exploited as diagnostic genetic markers
  • has knowledge of fundamental themes within functional genomics and proteomics
  • has broad knowledge about the principles underlying central laboratory methods employed in molecular biology, e.g. extraction of nucleic acids, PCR, cloning, melting point analysis, electrophoresis and DNA sequencing
  • has knowledge about central molecular biological laboratory methods are employed in diagnostics and in the development of treatment therapies
  • has knowledge about different types of genetic testing, gene therapy and cloning
  • is familiar with laws and regulations that govern genetic testing and genetic counselling
Ferdigheter

The student

  • can apply central methods in molecular biology such as nucleic acid extraction, quantitation, restriction, PCR-amplification (end point, semiquantitative and quantitative), dissociation, hybridisation sequencing and cloning
  • master interpretation of human pedigrees displaying the inheritance pattern of traits, syndromes and disease states 
Generell kompetanse

The student

  • can plan and carry out experiments involving genetic material and chemicals while observing all laboratory safety routines and procedures in force for waste treatment  
  • has insight into the provisions of Norway’s Gene Technology Act and the security measures for handling genetically modified organisms (GMO)
  • can present self-generated results in writing according to the IMRAD model
  • has insight into laws and regulations that govern genetic testing and genetic counselling
Arbeids- og undervisningsformer

Organisation and methods of instruction

  • lectures
  • laboratory exercises

The course can be taught in English if international students are enrolled.

Practice

Internal practice in form of laboratory exercises.

Arbeidskrav

Requirements necessary to take the exam

  • 100% attendance to seven - eight days of laboratory exercises
  • three to five individual written assignments from selected laboratory exercises (in English or in Norwegian)
Vurdering
  • 4-hour individual written school exam (in English or in Norwegian)
Vurderinger
VurderingsordningKarakterskalaGruppe/individuellVarighetHjelpemidlerAndelKommentar
Skriftlig eksamen med tilsyn
ECTS - A-F
Individuell
4 Time(r)
  • Ingen hjelpemidler
  • Godkjent kalkulator

Pensum

Fakultet
Fakultet for anvendt økologi, landbruksfag og bioteknologi
Institutt
Institutt for bioteknologi