BIOING1202 Molecular biology and genetics
- EmnekodeBIOING1202
- Studiepoeng10
- Undervisningssemestre2023 Høst
- UndervisningsspråkNorsk/Engelsk
- UndervisningsstedHamar
- Forkunnskapskrav
Studenten må normalt ha fullført minst 45 studiepoeng i løpet av det første studieåret i bachelor bioingeniørfag
Course content
- structure and function of RNA and DNA (both chromosomal and extrachromosomal), nucleoid, chromatin, chromosomes (organellar, and nuclear - both interphase, mitotic and meiotic) and extrachromosomal DNA
- chromosomal and extrachromosomal DNA replication, mutation, repair and recombination (site-specific, homologous, meiotic and somatic)
- prokaryotic and eukaryotic transcription and regulation thereof, including RNA processing in eukaryotes
- regulation of translation in prokaryotes and eukaryotes
- regulation of replication, transcription and translation of viruses that utilise prokaryotic or eukaryotic hosts
- genetic mutation, polymorphism, variation and inheritance, pedigree analysis, human Mendelian genetics, chromosomal aberrations and deviant states of ploidy
- recombinant DNA technology (including cloning), genetic engineering, genetically modified organisms, DNA sequencing, polymerase chain reaction (PCR, also quantitative PCR, QPCR), gene editing and gene therapy
- genome organisation, genomics, functional genomics, proteomics, transcriptomics
- epigenetics -- histone modification, DNA methylation, X-inactivation, DNA imprinting; prions and Creutzfeldt-Jakob disease
- regulations for work with biological material and GMO
- laboratory exercises, including nucleic acid extraction, quantitation, restriction, PCR-amplification (end point, semiquantitative and quantitative), dissociation, hybridisation sequencing and cloning; report writing with an emphasis on the analysis and dissemination of results and conclusions from analyses
Læringsutbytte
Upon passing this course, the graduate will have achieved the following learning outcomes:
The student
- has knowledge of the content, meaning and expression of genetic information from DNA to protein
- has knowledge of the regulatory mechanisms controlling DNA replication, transcription and translation in humans and organisms (including viruses) relevant for human health
- has knowledge about all forms of mutation of nucleic acids, how they arise, their effects on gene expression and how they are repaired
- has knowledge of the principles of genetic inheritance, pedigree analysis and how mutant alleles are inherited and how they can affect phenotypes including causing disease
- has knowledge about different forms of polymorphisms in DNA and how they can be developed and exploited as diagnostic genetic markers
- has knowledge of fundamental themes within functional genomics and proteomics
- has broad knowledge about the principles underlying central laboratory methods employed in molecular biology, e.g. extraction of nucleic acids, PCR, cloning, melting point analysis, electrophoresis and DNA sequencing
- has knowledge about central molecular biological laboratory methods are employed in diagnostics and in the development of treatment therapies
- has knowledge about different types of genetic testing, gene therapy and cloning
- is familiar with laws and regulations that govern genetic testing and genetic counselling
The student
- can apply central methods in molecular biology such as nucleic acid extraction, quantitation, restriction, PCR-amplification (end point, semiquantitative and quantitative), dissociation, hybridisation sequencing and cloning
- master interpretation of human pedigrees displaying the inheritance pattern of traits, syndromes and disease states
The student
- can plan and carry out experiments involving genetic material and chemicals while observing all laboratory safety routines and procedures in force for waste treatment
- has insight into the provisions of Norway’s Gene Technology Act and the security measures for handling genetically modified organisms (GMO)
- can present self-generated results in writing according to the IMRAD model
- has insight into laws and regulations that govern genetic testing and genetic counselling
Organisation and methods of instruction
- lectures
- laboratory exercises
The course can be taught in English if international students are enrolled.
Practice
Internal practice in form of laboratory exercises.
Requirements necessary to take the exam
- 100% attendance to seven - eight days of laboratory exercises
- three to five individual written assignments from selected laboratory exercises (in English or in Norwegian)
| Vurderingsordning | Karakterskala | Gruppe/individuell | Varighet | Hjelpemidler | Andel | Kommentar |
|---|---|---|---|---|---|---|
Skriftlig eksamen med tilsyn | ECTS - A-F | Individuell | 4 Time(r) |
|
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